Epidermolytic toxin from Staphylococcus aureus binds to filaggrins
- 6 January 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 194 (2) , 309-312
- https://doi.org/10.1016/0014-5793(86)80107-7
Abstract
The affinity of epidermolytic toxin from Staphylococcus aureus for proteins from the target tissue has been tested by a Western blotting procedure. Particular proteins in a l M phosphate extract of epidermis reacted on nitrocellulose blots with a probe prepared by the conjugation of toxin with peroxidase. Protein extracted into 50 mM Tris-HCl did not react. The probe detected profilaggrin, filaggrin and a smaller unidentified polypeptide. It is suggested that the interaction is relevant to the mode of action of the toxin.Keywords
This publication has 21 references indexed in Scilit:
- Identification of two intermediates during processing of profilaggrin to filaggrin in neonatal mouse epidermis.The Journal of cell biology, 1984
- Histidine-rich proteins (filaggrins): Structural and functional heterogeneity during epidermal differentiationJournal of Molecular Biology, 1983
- Histidine-rich protein of the keratohyalin granulesBiochimica et Biophysica Acta (BBA) - General Subjects, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Protein synthesis during fetal development of mouse epidermisDevelopmental Biology, 1977
- Desmosomes, Filaments, And Keratohyaline Granules: Their Role In The Stabilization And Keratinization of The EpidermisJournal of Investigative Dermatology, 1975
- Bovine Keratohyalin: Anatomical, Histochemical, Ultrastructural, Immunologic, And Biochemical StudiesJournal of Investigative Dermatology, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Coupling of enzymes to proteins with glutaraldehydeImmunochemistry, 1969
- PURIFICATION AND QUANTITATION OF GLUTARALDEHYDE AND ITS EFFECT ON SEVERAL ENZYME ACTIVITIES IN SKELETAL MUSCLEJournal of Histochemistry & Cytochemistry, 1967