A New Metabolic Labelling Medium for Trichomonas Vaginalis and Tritrichomonas Foetus Using 35S methionine
- 1 April 1986
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 181 (4) , 620-623
- https://doi.org/10.3181/00379727-181-4-rc1
Abstract
A metabolic labelling medium was devised for Trichomonas vaginalis and Tritrichomonas foetus utilizing 35S methionine. T. vaginalis cultured for 24h in the medium took up approximately 27% of the available label and increased greater than two fold in number. Counts per microgram of protein were 32,555 .+-. 10% between different strains or identical strains in different labelling runs. T. foetus took up approximately 5% of the available label and increased greater than two fold in 24h. This resulted in specific labelling of 12,704 cpm/.mu.g protein .+-. 10% between different runs with the same strain.This publication has 3 references indexed in Scilit:
- Interaction between Trichomonas vaginalis and other pathogenic micro-organisms of the human genital tract.Sexually Transmitted Infections, 1984
- Specific and common antigens of Trichomonas vaginalis detected by monoclonal antibodiesInfection and Immunity, 1984
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976