Application of Reversed-Phase HPLC in Separation and Detection of the Cyclodepsipeptide Toxins Produced by the Entomopathogenic Fungus Metarhizium anis

Abstract
A procedure is described for preparative separation of destruxins (cyclodepsipeptide toxins) produced in vitro and in vivo by the entomopathogen Metarhizium anisopliae. Methods for detection of microgram quantities of destruxins in fungal culture filtrates and insect blood are illustrated. Some examples of biological applications are also given.

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