Replication of plasmids from Staphylococcus aureus in Escherichia coli.

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Abstract
Plasmid pBR322 derives from plasmid ColE1 and does not replicate in E. coli strains lacking DNA polymerase I. Hybrids between pBR322 and a plasmid isolated from S. aureus, pC194, replicate in such E. coli strains, provided that the pC194 replication region is intact. Inactivation of the pBR322 replication region does not interfere with the replication of hybrids in E. coli. Hybrids between pBR322 and 2 other plasmids from S. aureus, pT127 and pUB112, replicate at the restrictive temperature in E. coli having thermosensitive DNA polymerase I. Similar hybrids involving pC221 and pHV400, plasmids from S. aureus and Bacillus subtilis, respectively, do not replicate under such conditions. Some plasmids from a gram-positive bacterium, S. aureus, can replicate in a gram-negative one, E. coli.