Abstract
Juices from viruliferous Macrosteles divisus Uhler were diluted to 10−1 and 10−3 with saline and 1/8000 cc per insect was injected into non-viruliferous leafhoppers. The infectivity tests of injected vectors under controlled environmental conditions showed that with a dilution of 10−1 the shortest incubation periods were from 11 to 15 days. With a dilution of 10−3 the minimal incubation periods were from 24 to 38 days. The results can be interpreted on the basis of the fact that multiplication of a small amount of virus would take a longer time to render the insects infective than multiplication of a greater amount of the aster-yellows virus.
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