Prostaglandin E2 potentiates the immunologically stimulated histamine release from human peripheral blood‐derived mast cells through EP1/EP3 receptors
- 27 February 2006
- Vol. 61 (4) , 503-506
- https://doi.org/10.1111/j.1398-9995.2006.01043.x
Abstract
Mast cells cultured from human peripheral blood have been widely used to study human mast cell function. Prostanoids are the important regulators of mast cell activity, however, there were no reports about the class of prostanoid receptors expressed on such cultured cells. The present study was to characterize pharmacologically the prostanoid receptors by investigating the effects of prostanoid receptor agonists on the immunoglobulin E (IgE)-mediated histamine release from the cultured mast cells. Mast cells cultured from human progenitor cells in peripheral blood were sensitized with human myeloma IgE, and then challenged with anti-human IgE following pretreatment with diverse prostanoid receptor agonists. The histamine content in supernatants and cell pellets were measured by histamine auto-analyzer. Of the prostanoid receptor agonists tested, the prostaglandin E2 (PGE2) receptor (EP receptor) agonist PGE2 (10(-7) to 10(-11) M) produced concentration-related potentiation of IgE-mediated histamine release from the cultured mast cells. Sulprostone, an EP1/EP3 agonist, SC-46275, a selective EP3 agonist, and 11-deoxy-PGE1, a selective EP2/EP3/EP4 agonist also caused a significant increase in histamine release induced by anti-IgE. BW245C, fluprostone, cicaprost and U46619 for the prostaglandin D2, F2alpha, I2, and thromboxane A2 receptors respectively, and the EP2/EP4 receptor agonist butaprost had little effect on anti-IgE stimulated histamine release from mast cells. The present results suggest that PGE2 potentiates the IgE-mediated histamine release from the cultured mast cell via EP3 and/or EP1 receptors.Keywords
This publication has 15 references indexed in Scilit:
- Prostaglandins E1 and E2 inhibit lipopolysaccharide-induced interleukin-18 production in monocytesEuropean Journal of Pharmacology, 2005
- Pharmacology and signaling of prostaglandin receptors: Multiple roles in inflammation and immune modulationPharmacology & Therapeutics, 2004
- New insights into the role of the mast cell in asthmaClinical and Experimental Allergy, 2003
- Contribution of the two Gs-coupled PGE2-receptors EP2-receptor and EP4-receptor to the inhibition by PGE2 of the LPS-induced TNFα-formation in Kupffer cells from EP2-or EP4-receptor-deficient mice. Pivotal role for the EP4-receptor in wild type Kupffer cellsJournal of Hepatology, 2002
- Selective down-regulation of high-affinity IgE receptor (FcεRI) α-chain messenger RNA among transcriptome in cord blood–derived versus adult peripheral blood–derived cultured human mast cellsBlood, 2001
- Ligand binding specificities of the eight types and subtypes of the mouse prostanoid receptors expressed in Chinese hamster ovary cellsBritish Journal of Pharmacology, 1997
- Histamine release and calcium concentrations in rat mast cells are dependent on intracellular ATP: Effects of prostaglandin D2Prostaglandins, Leukotrienes & Essential Fatty Acids, 1994
- SC-46275: A potent and highly selective agonist at the EP3 receptorProstaglandins, Leukotrienes & Essential Fatty Acids, 1993
- Characterization of the prostaglandin E receptor expressed on a cultured mast cell line, BNu-2c13Biochemical Pharmacology, 1993
- Two types of human mast cells that have distinct neutral protease compositions.Proceedings of the National Academy of Sciences, 1986