Determination of iota-carrageenan with 2-thiobarbituric acid

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Abstract
A colorimetric method for the determination of iota-carrageenan is described, based on the measurement of the yellow colour developed by the reaction of the 3,6-anhydro-D-galactose residue with 2-thiobarbituric acid and hydrochloric acid. The method has a sensitivity equivalent to 0·01 mg ml–1 of carrageenan and can also be used to determine carrageenan in blood and urine in concentrations of 0·02 and 0·01 mg ml–1, respectively. The method has advantages of sensitivity, relative specificity, a small sample-volume requirement and convenience over previously published methods for the determination of sulphated polysaccharides, thus providing a means of extending quantitative biological investigations of this group of substances.

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