The proteome of Saccharomyces cerevisiae mitochondria
Top Cited Papers
- 23 October 2003
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 100 (23) , 13207-13212
- https://doi.org/10.1073/pnas.2135385100
Abstract
We performed a comprehensive approach to determine the proteome of Saccharomyces cerevisiae mitochondria. The proteins of highly pure yeast mitochondria were separated by several independent methods and analyzed by tandem MS. From >20 million MS spectra, 750 different proteins were identified, indicating an involvement of mitochondria in numerous cellular processes. All known components of the oxidative phosphorylation machinery, the tricarboxylic acid cycle, and the stable mitochondria-encoded proteins were found. Based on the mitochondrial proteins described in the literature so far, we calculate that the identified proteins represent ≈90% of all mitochondrial proteins. The function of a quarter of the identified proteins is unknown. The mitochondrial proteome will provide an important database for the analysis of new mitochondrial and mitochondria-associated functions and the characterization of mitochondrial diseases.Keywords
This publication has 59 references indexed in Scilit:
- 'Omics' of the mitochondrionNature Biotechnology, 2003
- MitochondriaCell, 2003
- Insertion of Hydrophobic Membrane Proteins into the Inner Mitochondrial Membrane—A Guided TourJournal of Molecular Biology, 2003
- Functional profiling of the Saccharomyces cerevisiae genomeNature, 2002
- Systematic screen for human disease genes in yeastNature Genetics, 2002
- Subcellular localization of the yeast proteomeGenes & Development, 2002
- Yeast Mitochondrial Dehydrogenases Are Associated in a Supramolecular ComplexBiochemistry, 2001
- Protein Import Channel of the Outer Mitochondrial Membrane: a Highly Stable Tom40-Tom22 Core Structure Differentially Interacts with Preproteins, Small Tom Proteins, and Import ReceptorsMolecular and Cellular Biology, 2001
- Life with 6000 GenesScience, 1996
- Mitochondria: beyond oxidative phosphorylationBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1995