Sex Differences in Kidney Morphology and Chloroform Necrosis

Publisher Website
Google Scholar
Abstract
The relation between kidney morphology and susceptibility to CHCl3 necrosis was studied in 4 groups of strain A mice: normal [female][female], normal [male][male], castrated [male][male] and testosterone-treated castrated [male][male]. The [male][male] were castrated at 5 wks. Testosterone was begun at 10 wks., 1 mg. in 0.25 ml. of sesame oil being inj. subcut. every other day for a total of 10 injns. At 13 wks. all mice were given 0.005 ml. of 12% CHC13 in olive oil/g. body wt. by stomach tube. 24 hrs. later 2 of 5 testosterone castrates were dead and the other 3 and all 5 normal [male][male] moribund. All surviving 24 hrs. after giving CHCl3 were killed and the kidneys sectioned and stained with hematoxylin and eosin and with Mallory''s aniline blue. There was extensive necrosis of parts of the proximal and distal convoluted tubules in normal [male][male] and in testosterone [male] castrates and no necrosis in [female][female] and in castrated [male][male]-. No necrosis occurred in Bowman''s capsules or in the upper parts of the proximal convoluted tubules.