A Comparison of the Genomes of Polioviruses by cDNA:RNA Hybridization

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Abstract
RNA from 11 strains of the 3 serotypes of poliovirus were isolated from infected HeLa [human cervical carcinoma] cells and hybridized with representative, radioactive complementary DNA (cDNA) from either poliovirus type 1 or type 2. Hybridization and thermal denaturation were analyzed by hydroxyapatite chromatography. cDNA to either poliovirus type 1 or 2 is competent to detect about 70-80% of the sequences in RNA from heterotypic strains and > 92% of the RNA sequences in homotypic strains. The 3 polio serotype genomes appear to have diverged from each other by .apprx. 20%. Both poliovirus type 1 and type 2 representative cDNA can be used for the detection of poliovirus genomic sequences in RNA isolated from tissue.