HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TOCOPHEROLS AND TOCOTRIENOLS AND ITS APPLICATION TO DIETS AND PLASMA OF FINNISH MEN .2. APPLICATIONS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 54  (1) , 41-46
Abstract
The composed 1-day diets and plasma of 40 Finnish men screened for a Se supplementation study were analyzed for tocopherols and tocotrienols. The men were divided into a low-Se group (in the screening phase plasma Se levels < 70 .mu.g/l and plasma .alpha.-tocopherol levels < 1.2 mg/100 ml) and a high-Se group (plasma Se > 70 .mu.g/l, plasma .alpha.-tocopherol not determined before the study). In the low-Se group plasma levels of .alpha.-tocopherol averaged 0.97 .+-. 0.18 mg/100 ml. The daily dietary intake of .alpha.-tocopherol was 6.1 .+-. 2.7 mg and that of total vitamin E 7.3 .+-. 3.1 mg of .alpha.-tocopherol equivalents. In the high-Se group the corresponding average values were 1.16 .+-. 0.21 mg of .alpha.-tocopherol/100 ml of plasma, 8.8 .+-. 4.3 mg of .alpha.-tocopherol/day and 10.3 .+-. 5.1 mg of .alpha.-tocopherol equivalents/day. The overall average for the contribution of .alpha.-tocopherol to the total dietary tocopherols was 44.6 .+-. 11.0%. In the plasma samples .alpha.-tocopherol accounted for 92.0 .+-. 2.1%, .beta.-tocopherol for 2.7 .+-. 0.7% and .gamma.-tocopherol for 5.3 .+-. 2.1% of the total amount of tocopherols.