Phosphorylation of Influenza Virus Nucleoprotein in vivo

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Abstract
Two-dimensional analysis of polypeptides from A/FPV/Rostock/34 (FP/R)-infected chick embryo fibroblast cells using nonequilibrium pH gradient gel electrophoresis followed by polyacrylamide gel electrophoresis showed that nucleoprotein (NP) was the only detectable virus phosphoprotein and was present in both the nucleus and cytoplasm. The kinetics of accumulation of phosphorylated NP in the nucleus and cytoplasm were similar, suggesting that the presence or absence of phosphate groups did not control the entry of NP into the nucleus. Two-dimensional analysis of [35S]methionine-labeled FP/R-infected cells revealed some major differences from previously published work which are discussed.