XV. Yeast sequencing reports. Isolation and DNA sequence of the STE13 gene encoding dipeptidyl aminopeptidase

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Abstract
We have isolated a mutant which exhibits partial constitutivity for a‐specific gene expression in α cells. The wild‐type gene was cloned and demonstrated to be allelic to the STE13 gene, which encodes the dipeptidyl aminopeptidase involved in processing of the α‐factor prepropheromone. Thus, the mating defect of the ste13 mutations in α cells may result both from the production of incompletely processed α‐factor and from the increased expression of a‐specific genes. The STE13 open reading frame of 931 amino acids contains a putative membrane‐spanning segment near its amino terminus and is 31% identical to a second yeast dipeptidyl aminopeptidase (DAP2). A null mutant of STE13 has been constructed: it is viable and sporulation‐proficient. The sequence has been deposited in the GenBank data library under Accession Number L21944.