A plaque assay for the study of influenza virus inhibitors

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Abstract
A plaque assay for influenza viruses in primary chick cell cultures was used to examine the inhibitory effects of ribavirin and cyclooctylamine. Influenza virus strains were uniformly sensitive to inhibition by ribavirin, but they varied greatly in their sensitivity to cyclooctylamine. By picking plaques, it was possible to select cyclooctylamine-resistant variants of predominantly sensitive strains. Recombination experiments showed that cyclooctylamine-resistance was not necessarily associated with either the haemagglutinin or the neuraminidase of influenza virus.