THE ACTION OF ACTINOMYCIN D AND DIEPOXYBUTANE ON RECOMBINATION OF TWO CLOSELY LINKED LOCI INHORDEUM

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Abstract
The effects of applying aqueous solutions of each of actinomycin D (50 μg/ml) and diepoxybutane (0.04%) at the pre-meiotic stage of Hordeum vulgare L. Emend. Lam. were determined with respect to segregation and recombination of the mutants xeand an, closely linked in a region proximal to the centromere in chromosome 3. The solutions were injected into the spike area and the uppermost internode. Recombination in diepoxybutane-treated barley was approximately threefold that of the control for the region tested. A corressponding increase resulted from treatment with actinomycin D. Lethality to culms was considerably greater from the antibiotic than from the alkylating agent. Actinomycin D also reduced the frequency of expression, among progeny plants, of the albino (an) phenotype. Although the effect of the two chemicals on recombination was similar, differences in other effects were considered to indicate different modes of action in raising recombination levels.