Efficiency of Transplanting Normal, Zona-Free, and Chimeric Embryos to One and Both Uterine Horns of Inbred and Hybrid Mice12

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Abstract
Normal (zona intact), zona-free, and chimeric mouse embryos were cultured overnight and 1053 were transplanted to either one or both uterine horns of 106 pseudopregnant inbred and hybrid hosts. A higher percentage of transplanted embryos developed successfully when transplanted to hybrid hosts than to inbred hosts. Transplanting to both horns yielded a higher percentage developing and fewer resorptions (“moles”) than transplanting to only one horn. Removal of the zona pellucida with pronase reduced the percentage of mice developing compared with intact embryos. Zona-free chimeric embryos were at least as viable as zona-free regular embryos.