Monoclonal Antibodies against Human Fibroblast Interferon

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Abstract
A stable hybridoma clone derived by fusion of mouse myeloma cells (cell line Fo) and spleen cells of immunized mice was isolated which secretes monoclonal antibodies against human fibroblast interferon (interferon-.beta.). The antibody inhibits the antiviral activity of human fibroblast interferon in an antiviral assay using human FS4 fibroblast cells, reacts immunologically with interferon-.beta. separated by sodium dodecylsulfate/polyacrylamide gel electrophoresis and subsequent transfer to nitrocellulose and adsorbs interferon-.beta. immunologically when bound to CNBr-activated Sepharose. It also inhibits the antiviral activity of human fibroblast interferon-.beta. from which the sugar moiety was cleaved off by enzymatic treatment. The antibody is probably directed against the protein moiety of the interferon molecules.