Expression in Escherichia coli of catalytically active phenylalanine ammonia‐lyase from parsley
Open Access
- 4 December 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 258 (2) , 335-338
- https://doi.org/10.1016/0014-5793(89)81687-4
Abstract
In parsley (Petroselinum crispum), phenylalanine ammonia‐lyase (PAL) is encoded by 4 structurally similar genes. The nucleotide sequence of a near full‐length cDNA and the deduced amino acid sequence of PAL‐4 are presented and compared with the corresponding sequences of PAL‐1, a previously described representative of the gene family. Transformation of Escherichia coli cells with PAL‐1 or PAL‐4 cDNA yielded catalytically active PAL, suggesting that the catalytic center of the enzyme is formed spontaneously rather than by a plant‐specific mechanism.Keywords
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