Molecular Cloning, Sequencing, and Expression of a cDNA Encoding -Glucosidase from Mucor javanicus

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Abstract
A cDNA encoding Mucor javanicus α-glucosidase was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 2,751 bp, and included an open reading frame which encodes a polypeptide of 864 amino acid residues with a molecular mass of 98,759 Da. The deduced ami no acid sequence showed homology to fungal and mammalian α-glucosidases and related enzymes, and the sequence around the putative active site was well conserved among these enzymes.The cloned gene was expressed in Escherichia coli cells to produce the α-glucosidase, which hydrolyzes not only maltose, but also soluble starch.

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