In Vitro Transposition System for Efficient Generation of Random Mutants of Campylobacter jejuni
Open Access
- 1 April 2001
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (7) , 3256-3260
- https://doi.org/10.1128/jb.183.7.2384-2388.2001
Abstract
Tn5 insertion mutants defective in regulation of thePseudomonas putida trpE and trpGDCoperons by tryptophan were found to contain insertions in the P. putida miaA gene, whose product (in Escherichia coli) modifies tRNATrp and is required for attenuation. Nucleotide sequences upstream of trpE andtrpG encode putative leader peptides similar in sequence to leader peptides found in other bacterial species, and the phenotypes of the mutants strongly suggest that transcription of these operons is regulated solely by attenuation.Keywords
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