Block Staining of Botanical Materials

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Abstract
Plant materials, including coleus stem tips, Psilotum stems and onion root tips, were stained in iron-mordanted celestine blue and safranin (Gray and Pickle 1956) prior to embedding and sectioning. Mitotic figures as well as general morphological and anatomical features are adequately stained by this procedure. The plant materials, subdivided so that the largest dimension is about 8-10 mm, are stained for 12-48 hr. The time is dependent upon the tissue and dilution of the stain used. Excess stain is washed out and the tissues are dehydrated, embedded, sectioned and mounted on slides in the usual manner. Following removal of the wax by xylene the sections may be counterstained, or a cover slip may be added immediately.

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