Abstract
The incorporation of acetate into both lipid and water soluble compounds, catalyzed by chloroplast preparations, was stimulated by ATP and CoA. In the presence of ATP and CoA, the absence of either glucose-6-phosphate or NADP resulted in the formation of water soluble compounds almost exclusively. Increasing concentration of either glucose-6-phosphate or NADP, in the presence of saturating amounts of the other, shifted the incorporation of acetate in favor of lipid. Mn and Mg ions stimulated the incorporation of acetate into lipid and water soluble compounds, but Mn was superior in stimulating lipid synthesis. Illumination of the chloroplast suspension during acetate metabolism favored the incorporation into lipid and simultaneously caused an apparent inhibition of incorporation into water soluble compounds. Of the latter, incorporation into the amino-acid fraction was most affected by illumination. Glutathione and mercaptoethanol, but not ascorbic acid, stimulated the incorporation of acetate into long-chain fatty acids. The sulfhydryl compounds stimulated the synthesis of saturated fatty acids more than that of the unsaturated fatty acids.