All-vegetable Protein Mixtures for Human Feeding

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Abstract
The results of the present study indicate that in the dog and under the conditions used, the cottonseed pigment, gossypol, is excreted in the feces almost quantitatively, particularly for total gossypol. Fecal free gossypol is about 3.5 times greater than intake, suggesting that some of the bound gossypol is liberated during passage through the gastrointestinal tract. The pigment was identified by maximal absorption curves, for the original compound and as the dianilido gossypol and phloroglucinol derivatives. Other chemical and biological tests also indicated that the fecal pigment is gossypol. Furthermore, typical gossypol reactions could not be demonstrated in fecal extracts of dogs fed cottonseed-free diets, and upon gossypol additions to the diet gossypol appeared in the feces. Several possible dietary factors were studied to learn the conditions under which gossypol is excreted with the feces. None of the factors studied, such as type and intake of calories, and different fats, altered the pattern of gossypol excretion in feces.

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