Structural organization of the two main rDNA size classes ofAscaris lumbricoides
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (3) , 1313-1332
- https://doi.org/10.1093/nar/12.3.1313
Abstract
The two main rDNA size classes in the genome of Ascaris lumbricoides consist of 8.8 kb and 8.4 kb long repeating units present in a quantitative ratio of roughly 10:1. They both contain the genes coding for 18 , 5.8S and 26S ribosomal RNAs. The length heterogeneity is due to a 450 bp long spacer region localized in the longer repeating unit which begins 870 bp upstream of the 5'-end 18S gene. A few additional microheterogeneities in base sequence occur at the 5'-end of the 26S gene. The 18S, 5.8S and 26S coding regions have been mapped on both the 8.8 kb and 8.4 kb repeating units and the localization of the 5'- and 3'-ends of the 18S and 26S genes has been performed by S1 protection. No intervening sequences are present in either coding region of the two main rDNA size classes.Keywords
This publication has 41 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- The ribosomal DNA of Calliphora erythrocephala; an analysis of hybrid plasmids containing ribosomal DNAJournal of Molecular Biology, 1980
- Extrachromosomal ribosomal RNA genes in Tetrahymena structure and evolutionJournal of Molecular Biology, 1979
- Changes in the abundance of polyadenylated RNA during slime mould development measured using cloned molecular hybridization probesJournal of Molecular Biology, 1979
- An intervening sequence in the gene coding for 25S Ribosomal RNA of Tetrahymena pigmentosaCell, 1979
- The genes for 18S, 5.8S and 28S ribosomal RNA of Bombyx mori are organized into tandem repeats of uniform lengthGene, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Ribosomal RNA genes in germ line and somatic cells of Ascaris lumbricoidesDevelopmental Biology, 1974
- Molecular aspects of chromatin elimination in Ascaris lumbricoidesDevelopmental Biology, 1972
- A membrane-filter technique for the detection of complementary DNABiochemical and Biophysical Research Communications, 1966