Isolation of α‐Terpineol Dehydratase from Pseudomonas gladioli
- 1 January 1992
- journal article
- Published by Wiley in Journal of Food Science
- Vol. 57 (1) , 241-244
- https://doi.org/10.1111/j.1365-2621.1992.tb05464.x
Abstract
An enzyme which converts the citrus by‐product limonene to α‐terpineol was purified 13‐fold. After cell disruption of P. gladioli, α‐terpineol dehydratase (α‐TD) remained associated with a heterogeneous group of particulate material. α‐TD was partially solubilized by extraction with 10 mM HEPES buffer, pH 7.0 containing 2.0% (w/v) Triton X‐100 and 0.5M sodium trichloroacetate. Two soluble forms existed in 1.0% (w/v) Triton X‐100 with apparent molecular weights of 94,500 and 206,500 daltons.Keywords
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