Characterization of the physiological substrate for lipopolysaccharide heptosyltransferases I and II
Open Access
- 1 August 2001
- journal article
- other
- Published by SAGE Publications in Innate Immunity
- Vol. 7 (4) , 263-270
- https://doi.org/10.1177/09680519010070040701
Abstract
L- Glycero-D- manno-heptopyranose is a characteristic compound of many lipopolysaccharide (LPS) core structures of Gram-negative bacteria. In Escherichia coli two heptosyltransferases, namely WaaC and WaaF, are known to transfer L- glycero-D- manno-heptopyranose to Re-LPS and Rd 2-LPS, respectively. It had been proposed that both reactions involve ADPL- glycero-D- manno-heptose as a sugar donor; however, the structure of this nucleotide sugar had never been completely elucidated. In the present study, ADPL- glycero-D- manno-heptose was isolated from a heptosyltransferase-deficient E. coli mutant, and its structure was determined by nuclear magnetic resonance spectroscopy and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry as ADPL- glycero-β-D- manno-heptopyranose. This compound represented the sole constituent of the bacterial extract that was accepted as a sugar donor by heptosyltransferases I and II in vitro .Keywords
This publication has 26 references indexed in Scilit:
- Efficient Chemical Synthesis of the Two Anomers of ADP-L-glycero- andD-glycero-D-manno-Heptopyranose Allows the Determination of the Substrate Specificities of Bacterial HeptosyltransferasesPublished by Wiley ,2000
- The rfaE Gene from Escherichia coli Encodes a Bifunctional Protein Involved in Biosynthesis of the Lipopolysaccharide Core Precursor ADP- l - glycero - d - manno -HeptoseJournal of Bacteriology, 2000
- Involvement of waaY, waaQ, and waaP in the Modification of Escherichia coliLipopolysaccharide and Their Role in the Formation of a Stable Outer MembraneJournal of Biological Chemistry, 1998
- Identification of a Novel Heptoglycan of α1→2-Linkedd-glycero-d-manno-HeptopyranoseJournal of Biological Chemistry, 1998
- Enzymatic Synthesis of Lipopolysaccharide in Escherichia coliJournal of Biological Chemistry, 1998
- Deletion of the Heptosyltransferase Genes rfaC and rfaF in Escherichia Coli K‐12 Results in an Re‐Type Lipopolysaccharide with a High Degree of 2‐Aminoethanol Phosphate SubstitutionEuropean Journal of Biochemistry, 1997
- Biosynthesis of Inner Core Lipopolysaccharide in Enteric Bacteria Identification and Characterization of a Conserved Phosphoheptose IsomerasePublished by Elsevier ,1996
- Structural analysis of two oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant strain of Escherichia coli F515 (Re chemotype) expressing the genus‐specific epitope of Chlamydia lipopolysaccharideEuropean Journal of Biochemistry, 1993
- Separation and quantification of ADP-d-glycero-d-mannoheptose and ADP-l-glycero-d-mannoheptose by borate liquid chromatography and its use in the assay of ADP-d- glycero-d-mannoheptose 6-epimeraseJournal of Chromatography A, 1986
- Lipopolysaccharide and Aldoheptose Biosynthesis in Transketolase Mutants of Salmonella typhimuriumProceedings of the National Academy of Sciences, 1971