Antiproliferative effect of hu-interferon-gamma in 674V and J82 bladder carcinoma cell lines

Abstract
Hu-IFN-gamma was evaluated in regard to the antiproliferative effect on J82 and 647V bladder cancer cell lines. In addition, the IFN-receptors were determined. There was a significant growth inhibition of J82 as well as 647V at low dose Hu-IFN-g (1 U/ml). The growth inhibition was significantly higher in 647V than in J82. The binding assay for 125J-Hu-IFN-g revealed 870 and 3,000 binding sites for 647V and J82, respectively, indicating that the antiproliferative effect of Hu-IFN-g may not depend on the absolute amount of IFN-receptors, in the two cell lines tested.

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