Abstract
After dark adaptation of oxygen‐evolving PS II complexes, oscillatory absorption changes (stable > 0.5 s) with a periodicity of 4 were detected at 514 nm. They have been correlated with the four oxidation states of the water‐splitting enzyme system S. Supposing that the changes are due to electrochromic shifts, they might indicate a positive surplus charge in states S2and S3. This means that the electron release pattern 1:1:1:1 is accompanied by a pattern of charge formation 0: + : + : 0 and an intrinsic proton release stoichiometry 1:0:1:2 for the transitions So→S1, S1→S2, S2→S3, S3→S0.

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