Limited Infection of Cereal Leaf Protoplasts by Barley Yellow Dwarf Virus

Abstract
Oat and barley leaf protoplasts that were readily infected with brome mosaic virus could only be infected with barley yellow dwarf virus (BYDV) at low levels of efficiency. Of the conditions tested for infecting the protoplasts with BYDV, the most reliable combination was 2 .mu.g/ml of virus and 0.2 .mu.g/ml poly-L-ornithine with 10 mM-citreate buffer at pH 5. Tobacco protoplasts could not be infected with BYDV under these conditions.

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