Identification of spliced RNA species ofDrosophila melanogaster gypsyretrotransposon New evidence for retroviral nature of thegypsyelement
- 15 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 350 (1) , 147-150
- https://doi.org/10.1016/0014-5793(94)00758-6
Abstract
We have identified a novel RNA species of Drosophila melanogaster gypsy retrotransposon that is ca. 2 kb in length and corresponds to the third open reading frame (ORF3) of the gypsy element. This RNA is generated by splicing of the primary gypsy transcript, as is the case for retroviral env gene expression. Therefore, the striking resemblance between gypsy and retroviruses has now been extended by this study to the expression strategies of these retroelements. The primary structure of spliced RNA was determined, and its analysis shows that both gypsy subfamilies (6K and 7K) apparently are able to encode functionally active ORF3 translation products.Keywords
This publication has 11 references indexed in Scilit:
- Retroviruses in invertebrates: the gypsyretrotransposon is apparently an infectious retrovirus of Drosophilamelanogaster.Proceedings of the National Academy of Sciences, 1994
- The introduction of a transpositionally active copy of retrotransposon GYPSY into the Stable Strain of Drosophila melanogaster causes genetic instabilityMolecular Genetics and Genomics, 1994
- TwoDrosophilaretrotransposongypsysubfamilies differ in ability to produce new DNA copies via reverse transcription inDrosophilacultured cellsNucleic Acids Research, 1993
- Splicing signals inDrosophila: intron size, information content, and consensus sequencesNucleic Acids Research, 1992
- Molecular analysis of the gypsy (mdg4) retrotransposon in two Drosophila melanogaster strains differing by genetic instabilityMolecular Genetics and Genomics, 1990
- TRANSCRIPTION AND REVERSE TRANSCRIPTION OF RETROTRANSPOSONSAnnual Review of Microbiology, 1989
- The Drosophila melanogaster gypsy transposable element encodes putative gene products homologous to retroviral proteins.Molecular and Cellular Biology, 1986
- The steps of reverse transcription of drosophila mobile dispersed genetic elements and U3-R-U5 structure of their LTRsCell, 1986
- Structural organization of transposable element mdg4 fromDrosophila melanogaslerand a nucleotide sequence of its long terminal repeatsNucleic Acids Research, 1984
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977