Free and Immobilized Glucose Isomerase from Streptomyces phaeochromogenes1
Open Access
- 1 January 1971
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 21 (4) , 588-593
- https://doi.org/10.1128/aem.21.4.588-593.1971
Abstract
Properties were determined of the glucose isomerase from Streptomyces phaeochromogenes NRRL B-3559. The enzyme exhibited a temperature optimum of 80 C and a pH optimum of about 8. The effect of various buffers on activity of the enzyme and the optimum pH were studied. Michaelis constants for glucose and Mg2+ were 0.25 and 0.025 m, respectively. Co2+ enhanced enzyme activity. A functional polyacrylamide-entrapped glucose isomerase was prepared. The conditions for entrapment and use of the bound enzyme were examined.Keywords
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