Large-Scale Purification of Na,K-ATPase and Its Protein Subunits from Lamb Kidney Medulla
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 9 (2) , 157-170
- https://doi.org/10.1080/00327487908061681
Abstract
Procedures are described for the large-scale isolation of purified Na,K-ATPase (EC 3.6.1.3) from frozen lamb kidney outer medulla and for the separation of its two protein subunits by hy-droxyapatite chromatography in sodium dodecyl sulfate (SDS). The methods described permit the routine isolation of up to 800 mg of purified Na,K-ATPase in one week, which can subsequently be separated into 500 mg of Mr= 95,000 catalytic subunit and 200 mg of glycoprotein with four SDS-hydroxyapatite column runs.This publication has 17 references indexed in Scilit:
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