Opioid Peptides Determination by Tyrosinase-Modified Oxygen Electrode
- 1 October 1995
- journal article
- chemical and-bio-sensors
- Published by Taylor & Francis in Analytical Letters
- Vol. 28 (13) , 2297-2304
- https://doi.org/10.1080/00032719508000373
Abstract
Opioid peptides morphiceptin (Tyr-Pro-Phe-Pro-NH2), [D-Ala2, D-Leu5]-enkephalin (Tyr-D-Ala-Gly-Phe-D-Leu, DADLE) and [D-Thr2]-Leu-enkephalin-Thr (Tyr-D-Thr-Gly-Phe-Leu-Thr, DTLET) containing tyrosine has been studied in the reaction with mushroom tyrosinase immobilized on Clark-type oxygen electrode. A 4–6-times higher response is observed for tyrosine compared to peptides. The detection limit of the tyrosinase-modified electrode for DADLE, morphiceptin and DTLET was 6 μM, 9 μM and 16 μM, respectively.Keywords
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