METHODS FOR ISOLATING, COLLECTING AND ORIENTING SALIVARY GLAND CHROMOSOMES FOR DIFFRACTION ANALYSIS
- 1 May 1942
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Heredity
- Vol. 33 (5) , 173-184
- https://doi.org/10.1093/oxfordjournals.jhered.a105162
Abstract
Two methods for isolating salivary gland nuclei from unfixed glands and three methods for isolating nuclei and single chromosomes from fixed glands are described. Techniques for collecting these nuclei and chromosomes and mounting them in various ways for X-ray and electron diffraction photography are described. The photographs taken to date have not justified any definite conclusions regarding the structure of salivary gland chromosomes, but the technique has not been adequately tested. It is pointed out that the methods may be of value in physiological and biochemical investigations, and in electron microscopy.This publication has 0 references indexed in Scilit: