An exonuclease possibly involved in the initiation of repair of bleomycin-damaged DNA in mouse ascites sarcoma cells
- 1 December 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 9 (12) , 2239-2244
- https://doi.org/10.1093/carcin/9.12.2239
Abstract
A protein factor having exonucleolytic activity on bleomycindamaged DNA and providing priming sites for DNA polymerasesexisted in a DNA polymerase β fraction partially purified by ion exchange chromatography from an extract of permeable mouse ascites sarcoma (SR-C3H/He) cells. The exonuclease was separated from DNA polymerase β by singlestranded DNA-cellulose chromatography, and partially characterized. The enzyme is suggestedto be involved in the initial step of repair of bleomycin-damaged DNA in removing 3′ ends (3′-phosphoglycolate termini) of bleomycin-damaged DNA.Keywords
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