Noncovalent Interactions: Defining Cooperativity. Ligand Binding Aided by Reduced Dynamic Behavior of Receptors. Binding of Bacterial Cell Wall Analogues to Ristocetin A
- 23 January 2004
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (7) , 2042-2049
- https://doi.org/10.1021/ja039409p
Abstract
Changes in the relative populations of the monomer and asymmetric dimer forms of ristocetin A, upon binding of two molecules of ligand, suggest that ligand binding is negatively cooperative with respect to dimerization. However, strong hydrogen bonds formed in the binding sites of the ligands are reinforced in the dimer relative to the monomer, and the barrier to dissociation of the dimer is increased upon binding of the ligands. It is concluded that the interactions which are common in the binding of both ligands are made with positive cooperativity with respect to those involved in dimerization. The conclusions are relevant to the binding of ligands to proteins, where ligand binding energy can be derived from stabilization of the protein in its ligand-bound form.Keywords
This publication has 15 references indexed in Scilit:
- Ligand Binding Energy and Catalytic Efficiency from Improved Packing within Receptors and EnzymesJournal of Molecular Biology, 2003
- An Enthalpic Component in Cooperativity: The Relationship between Enthalpy, Entropy, and Noncovalent Structure in Weak AssociationsJournal of the American Chemical Society, 2001
- The propagation of binding interactions to remote sites in proteins: Analysis of the binding of the monoclonal antibody D1.3 to lysozymeProceedings of the National Academy of Sciences, 1999
- Conformational Change in the Human Glucocorticoid Receptor Induced by Ligand Binding Is Altered by Mutation of Isoleucine 747 by a ThreonineJournal of Biological Chemistry, 1999
- Elucidation of the Chain Conformation in a Glassy Polyester, PET, by Two-Dimensional NMRScience, 1998
- Thermodynamics and kinetics of dissociation of ligand-induced dimers of vancomycin antibioticsJournal of the Chemical Society, Faraday Transactions, 1997
- Aspects of molecular recognition: solvent exclusion and dimerization of the antibiotic ristocetin when bound to a model bacterial cell-wall precursorJournal of the American Chemical Society, 1989
- Hydrogen bond length and proton NMR chemical shifts in proteinsJournal of the American Chemical Society, 1983
- The specificity of combination between ristocetins and peptides related to bacterial cell wall mucopeptide precursorsBiochemical Journal, 1971
- On the nature of allosteric transitions: A plausible modelJournal of Molecular Biology, 1965