The kinetic mechanism of the major form of ox kidney aldehyde reductase with d-glucuronic acid

Abstract
The steady-state kinetics of the major form of ox kidney aldehyde reductase with D-glucuronic acid were determined at pH 7. Initial rate and product inhibition studies performed in both directions are consistent with a Di-Iso Ordered Bi Bi mechanism. The mechanism of inhibition by sodium valproate and benzoic acid is shown to involve flux through an alternative pathway.