The cloning and expression of a gene encoding haemolytic activity from the fish pathogen Renibacterium salmoninarum

1 September 1990

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 71 (1-2) , 31-34
https://doi.org/10.1016/0378-1097(90)90028-o

Abstract

A gene encoding haemolytic activity from Renobacterium salmoninarum (strain PPD)) was cloned into Escherichia coli using the cosmid vector pHC79, and subsequently subcloned on a 1.6 kbp SA /I fragment into pBR328. Southern blot hybridisation revealed that a homologous sequence is found in other strains of R. salmoninarum .

Keywords

BACTERIAL KIDNEY DISEASE
GENE CLONING
HAEMOLYTIC ACTIVITY

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