INDUCTION OF TRANSPLANTATION TOLERANCE IN GUINEA PIGS BY SPLEEN ALLOGRAFTS

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Abstract
Spleen allografts from [guinea pig] (2 .times. 13)F1 donors survive indefinitely im immunologically intact strain 2 recipients. Such strain 2 hosts with long-term surviving F1 grafts had markedly suppressed anti-13 MLR [mixed leukocyte reaction] responses and cells capable of suppressing the response of normal strain 2 cells to strain 13 stimulators were present in some long-term surviving animals. To further analyze the cellular basis for this tolerant state, cells were transferred from tolerant donors to normal syngeneic strain 2 hosts. Such suppressor cells transfer MLR unresponsiveness to primary or secondary hosts, but the period of MLR suppression is rather short-lived. Strain 13 cardiac allografts survived indefinitely in cell-transferred primary or secondary hosts and further enhanced MLR suppression, whereas most cardiac allografted controls had vigorous MLR responses for strain 13 stimulator cells after rejection of their transplants. Attempts to abrogate established tolerance in transferred hosts, either by donor antigen or by host cytolytic T cells were unsuccessful.