One Month of Streptozotocin-Diabetes Induces Different Neuroendocrine and Morphological Alterations in the Hypothalamo-Pituitary Axis of Male and Female Rats*

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Abstract
LHRH (median eminence) and LH [luteinizing hormone] (pituitary and plasma) from male and female Sprague-Dawley rats were assayed 1 mo. after steptozotocin injection and compared with values in controls either fed and ad libitum or offered a restricted diet. Plasma LH was also assayed after stimulation with exogenous LHRH or naloxone. In diabetic males, the median eminence LHRH content and the plasma LH response to exogenous LHRH were unaltered, pituitary LH was increased, and plasma LH was decreased under basal conditions and after naloxone treatment. In diabetic females, while the median eminence LHRH content and the plasma LH response to exogenous LHRH or naloxone were reduced, pituitary and plasma LH levels were not different. Measurements made in undernourished rats excluded the possibility that the alterations found in diabetic animals were nutrition dependent. In parallel experiments, hypothalami and pituitaries were examined morphologically. In diabetic animals, degenerate axons, mainly of the LHRH type, were found in the arcuate nucleus and median eminence, and LH gonadotrophs were altered and more numerous. Stong differences between control males and females were revealed by morphometry; diabetic females had higher brain wt and fewer LH gonadotroph changes than diabetic males. Apparently, the hypothalamo-pituitary changes that occur early in the streptozotocin-treated rats are unrelated to undernourishment and are possibly caused by insulin deficiency; the LHRH axonal lesions might play a primary pathogenic role in the hypothalamo-pituitary disorder, some anatomical data indicate that the brain and pituitary are less severely affected by diabetes in female than in male animals; and differences between control males and females may account for some of the dissimilarities between the sexes observed under diabetic conditions.