A METHOD FOR THE ASSAY OF THE GONADOTROPIN CONTENT OF NORMAL HUMAN URINE1

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Abstract
The tannic acid, benzoic acid, and ethanol pptn. procedures with and without dialysis or 50% ethanol ex-tradtion were compared on normal and menopause urines and on urines to which known amounts of urinary gonado-trophin had been added. The crude ethanol or tannic acid precipitates from normal human urine were too toxic to be assayed on immature [female] mice. The toxicity was incompletely removed and the activity poorly recovered by dialysis of these precipitates. The benzoic acid pptn. procedure led to very poor recovery. Extraction of the crude ethanol precipitate by 50% ethanol and reprecipitation by 4 vols. of ethanol eliminated the toxicity satisfactorily and gave a 75% recovery of the original urine gonadotrophin content.