Packing High Performance Liquid Chromatography Columns Using Organic Acids as Slurry Solvents

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Abstract
Agglomeration of particles in an HPLC packing slurry produces a catastrophic effect on column efficiency. The agglomerative phenomenon is believed to be caused by coulombic attraction forces between particies. Organic acids minimized the particle agglomeration by decreasing such attraction forces through protonation of gel surface negative sites. Column efficiency increased linearly with acid volume until a maximum value was reached. Two types of stable slurries were developed using specific organic acids as the slurry solvent. A combination of n-heptanoic acid and dichioroacetic acid was used to slurry reversed phase materials. Dichioroacetic acid was used to slurry normal-phase materials. These two system resulted in both stable slurries and reproducible, efficient, easily packed HPLC columns when used with a variety of commercial packing materials.

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