Measurement of Interleukin-6 Production by Monocytes for in Vitro Safety Testing of Hemoglobin Solutions
- 1 January 1994
- journal article
- research article
- Published by Taylor & Francis in Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
- Vol. 22 (3) , 835-840
- https://doi.org/10.3109/10731199409117918
Abstract
Induction of interleukin-6 (IL-6) production by isolated human mononuclear blood cells was taken as in vitro model for the induction of inflammatory reactions. The model was very sensitive to bacterial endotoxin (detection limit < 10pg/ml). Hemoglobin (Hb) solutions, prepared under non-sterile conditions also induced IL-6 production, which correlated with a positive reaction in the Limulus assay. Purification of the Hb solutions with a detergent prevented IL-6 production, showing that pure Hb itself does not activate the monocytes. We conclude that this assay is a useful and sensitive test of contamination with components that can induce inflammatory reactions, especially microbial products.Keywords
This publication has 3 references indexed in Scilit:
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- Removal of endotoxin from protein solutions by phase separation using triton X-114Journal of Immunological Methods, 1990
- Biocompatibility of Hemoglobin Solutions. II. The Inflammatory Reaction of Human Monocytes and Mouse Peritoneal MacrophagesArtificial Organs, 1990