Comparative Studies of Leaf Tissue and Isolated Mesophyll Protoplasts
- 1 February 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 31 (1) , 83-95
- https://doi.org/10.1093/jxb/31.1.83
Abstract
Rates of O2 exchange and of CO2 fixation by viable freshly isolated mesophyll protoplasts were similar to those of the original leaf tissue. Changes in pH and the addition of various uncouplers and inhibitors had similar effects on these processes in protoplasts and in leaf tissue. Prolonged incubation of the protoplasts and of the leaf tissue caused similar marked changes in their rates of O2 exchange. The results show that freshly isolated mesophyll protoplasts should provide a suitable system for the quantitative study of many of the physiological and biochemical processes of leaf cells.Keywords
This publication has 1 reference indexed in Scilit:
- Energy Relations of Phosphate Uptake and Distribution in Barley Leaf Slices as Affected by Cutting and Adaptive AgeingZeitschrift für Pflanzenphysiologie, 1976