Experimental infections by Bacteroides gingivalis in non‐immunized and immunized rabbits

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Abstract
The interactions between Bacteroides gingivalis and systemic antibodies were studied in tissue cages implanted in the backs of New Zealand white rabbits. Infectivity was evaluated according to clinical signs and to leukocyte and bacterial counts in material aspirated from the tissue cages. Pre- and post-inoculation antibody levels to sonicated whole bacterial cells were determined by enzyme-linked immunosorbent and agar immunodiffusion assays. Rabbits immunized against B. gingivalis and then challenged with pure cultures of B. gingivalis revealed complete elimination or markedly lower postinoculation bacterial counts and considerably weaker tissue reactions than non-immunized animals. B. gingivalis co-inoculated with Actinobacillus actinomycetemcomtans caused significantly more severe infections than observed in monoinfected animals. The present results suggest that the immune system acting through systemic antibodies and/or cellular mechanisms may modulate the pathogenic potential of infecting periodontal pathogens.