Calibration of transmission electron microscopic mass determination using objects with known mass distribution
- 1 April 1983
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 130 (1) , 85-92
- https://doi.org/10.1111/j.1365-2818.1983.tb04201.x
Abstract
SUMMARY: An improved calibration procedure is described for dry mass determination methods, that are based on microdensitometry of transmission electron micrographs. Polystyrene latex spheres, that are supposed to have a homogeneous mass distribution, are used for the calibration procedure. By computer fitting of the theoretically expected mass distribution to the distribution measured in a line through the centre of a sphere, a mass calibration factor is obtained. The number of measurements required for a predetermined calibration accuracy can be decreased considerably as compared to other calibration methods. Accurate calibration and reproducible adjustment of the electron optical magnification are required.Keywords
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