A point mutation in a mitocbondrial tRNA gene abolishes its 3′ end processing

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Abstract
A temperature sensitive mutation mapping in the tRNA region of the mitochondrial genome of Saccharomyces cerevisiae has been found to abolish 3'' processing of tRNAasp. Mutant cells grown for a few generations at the non-permissive temperature were found to specifically lack mature tRNAasp and to accumulate 3'' unprocessed precursors of this tRNA. The accumulation of precursors of other mitochondrial tRNAs was also observed under the same conditions. After longer incubation times, a generalized decrease of mitochondrial transcripts could be observed. The mutation was genetically mapped in a limited region surrounding the tRNAasp gene and found, by sequencing, to consist of a C->T transition at position 61 of the tRNAasp gene.

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