Specific DNA sequence amplification in human neuroblastoma cells.

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Abstract
Southern blot analysis of a number of EcoRI-digested human neuroblastoma DNA revealed the presence of a family of discrete restriction fragments, the majority of which are amplified in most, but not all, of the neuroblastoma cell lines tested. None of these sequences is abundantly present in DNA from other human tumors of different tissue origins, including several known or presumed to contain amplified DNA. These sequences appear to be specifically amplified by neuroblastoma cells. Hybridization with metaphase chromosomes in situ localized these sequences to the homogeneously staining regions or double-minute chromosomes of different neuroblastoma cell lines, indicating that these chromosomal structures, although present in cell lines established from different patients, share many sequences and may have a common, but as yet unknown, function.

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