A method for the spectrophotometric measurement of chlorophyll a and pheophytin a in benthic microalgae1
- 1 September 1975
- journal article
- research article
- Published by Wiley in Limnology and Oceanography
- Vol. 20 (5) , 887-896
- https://doi.org/10.4319/lo.1975.20.5.0887
Abstract
A method is described that allows the measurement of river periphyton chlorophyll a and pheophytin a both on rocks and in soft sediments. The method involves successive extractions directly from frozen samples with boiling methanol. Pigments are estimated by optical density measurements before and after acidification. Methanol is superior to acetone for the distinction of chlorophyll a and pheophytin a by this means.Keywords
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