Interaction Between Lymphocytes and Inflammatory Exudate Cells

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Abstract
Thymocytes of C3H/He mice were poorly triggered to induce DNA synthesis by phytohemagglutinin but fairly well triggered when they were mixed with small number of inflammatory peritoneal exudate polymorphonuclear leukocytes or macrophages, or with the soluble product(s) of these cells. Peritoneal exudate cells were mainly harvested at 3 hr, largely consisting of polymorphonuclear leukocytes, and at 96 hr, largely consisting of macrophages, after intraperitoneal injection of 0.2% sodium caseinate. Addition of 22 to 26 × 103 of 3-hr exudate cells, 22 to 24 × 103 of 48-hr exudate cells, or 22 to 24 × 103 of 96-hr exudate cells to syngeneic thymocyte culture (3 × 106) resulted in marked enhancement of DNA synthesis by thymocytes stimulated with 1 µ1 PHA. Similar enhancement of DNA synthesis was also observed in the presence of 200 µ1 of cellfree supernatant fluid of peritoneal exudates of 3 hr or 9 hr after casein stimulation. Conversely, two hundred microliters of 0-hr, 48-hr, or 96-hr peritoneal fluid showed a slight suppressing effect on thymocyte response and 50 to 100 µl of these exudates showed no enhancing effect. Furthermore, 50 to 800 µ1 of culture supernatants from 1.6 × 106 cells/4 ml of 3-hr exudate cells, 96-hr exudate cells, or purified macrophages also caused a similar enhancement of DNA synthesis by thymocytes. On the other hand, excess numbers of exudate cells (27 to 28 × 103 of 3-hr exudate cells, or 25 to 29 of 96-hr exudate cells) or excess amounts of culture supernatants (800 µl from 6.4 to 12.8 × 106/4 ml of 3-hr cells, or 200 µl from 12.8 × 106/4 ml, 400 µl from 6.4 × 106/4 ml, or 800 µl from 3.2 × 106/4 ml of 96-hr cells) diminished the enhancing potency on thymocytes. The cooperative function by polymorphonuclear leukocytes or by macrophages on the lymphocyte response was thus postulated.

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